What is one of the probe amplification methods?

Probe amplification methods are techniques that utilize specific probes to detect and amplify nucleic acid sequences. Ligase Chain Reaction (LCR) is a well-known method that specifically involves the use of DNA ligase and probes that hybridize adjacent to each other on a target DNA sequence. This method works by creating a ligated product that can then be amplified, allowing for the detection of specific sequences present in a sample.

In LCR, two complementary probes bind to the target sequence on either side of a region of interest. When these probes are close enough, the ligase enzyme facilitates the joining of the probes, creating a circular DNA molecule. This circular product can subsequently be amplified, resulting in an increased signal that can be detected, making it a sensitive method for quantifying specific nucleic acid sequences.

Other answer choices, while also important molecular biology techniques, do not classify strictly as probe amplification methods. Quantitative PCR primarily amplifies DNA without relying on a probe-based ligation mechanism for its quantitative analysis, Reverse Transcription PCR focuses on converting RNA to DNA before amplification, and Next Generation Sequencing allows for sequencing of DNA without discrete amplification based solely on ligation events. Thus, Ligase Chain Reaction stands out as a defined probe amplification technique.