What is referred to as a collection of DNA fragments prepared for sequencing?

The term that describes a collection of DNA fragments prepared for sequencing is a sequencing library. In the context of molecular biology, a sequencing library consists of a set of DNA fragments that have been prepared and are ready for sequencing processes. This preparation often involves the fragmentation of DNA, followed by the ligation of specific adapters to the ends of these fragments. These adapters are crucial for the sequencing machinery, as they allow the fragments to bind to the sequencing platform and facilitate the sequencing reactions.

Creating a sequencing library typically includes quality control steps to ensure that the size and concentration of the fragments are appropriate for the chosen sequencing technology. This process is essential in next-generation sequencing (NGS) methods where accurately sequencing libraries can significantly influence the eventual quality and reliability of the sequencing data obtained.

Other options do not accurately represent the concept of a collection of DNA fragments prepared for sequencing. For instance, a sequencing buffer refers to the solutions used during the sequencing process to maintain optimal conditions for the sequencing enzymes and reactions, but it is not a collection of DNA fragments. A sequencing plate could relate to formats where DNA libraries are formatted for sequencing but does not specifically define a library itself. A sequencing pool would imply a mixture or collection, but it lacks the specific context of preparation that a