What is the absorbance wavelength associated with phenol?

Prepare for the ASCP Molecular Biology (MB) Technologist Exam. Study with flashcards and multiple-choice questions, each with hints and explanations. Get ready to succeed!

Phenol has a characteristic absorption in the ultraviolet spectrum, particularly due to its aromatic ring structure, which can absorb UV light. The absorbance peak for phenol is typically observed around 270 nm. This is significant because the aromatic nature of phenol allows it to undergo π-to-π* transitions, which are responsible for its ability to absorb UV light at this specific wavelength.

In molecular biology and many biochemical assays, understanding the absorbance characteristics of various compounds is crucial for applications such as spectrophotometry. The peak at 270 nm is also useful when quantifying phenols in various samples, including those in biochemical procedures or environmental samples. Thus, the absorbance wavelength associated with phenol is accurately identified at this wavelength due to its unique electronic structure and transition properties.

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