What is the typical denaturation temperature in a PCR reaction?

In a PCR (Polymerase Chain Reaction) process, denaturation is a critical step where the double-stranded DNA is separated into two single strands. This part of the reaction requires sufficient heat to disrupt the hydrogen bonds holding the strands together. The typical denaturation temperature for most common DNA polymerases used in PCR is around 95°C.

This temperature is optimal because it reliably facilitates the melting of the DNA duplex without causing significant degradation of the nucleic acids or affecting the integrity of the polymerase enzyme. While some protocols may use temperatures ranging from 90°C to 98°C depending on specific experimental needs or the DNA structure being amplified, 95°C is a standardized choice that balances efficiency and effectiveness in ensuring that the DNA strands separate adequately for annealing and extension in subsequent steps of PCR.

In summary, 95°C is the most commonly utilized temperature for denaturation in PCR, making this answer the correct choice.