What probe is utilized in a Southern Blot technique?

In the Southern Blot technique, DNA probes are utilized to detect specific DNA sequences within a complex mixture. This method involves the separation of DNA fragments by gel electrophoresis, followed by transferring the fragments to a membrane and hybridizing them with labeled DNA probes that are complementary to the target sequence. The labeled probes bind specifically to their corresponding DNA sequences, allowing for their visualization and identification after washing away unbound probes.

DNA probes are crucial in this technique as they provide specificity in identifying the fragment of interest amid a multitude of DNA fragments present after gel electrophoresis. The binding of the probe to the target DNA can then be detected through various methods, often involving radioactivity or fluorescent labels. This makes DNA probes the correct choice for Southern Blot assays and emphasizes their role in molecular biology research and diagnostics.