Which amplification method is included in target amplification methods?

Transcription-Based Amplification is a form of target amplification that specifically focuses on increasing the quantity of a particular nucleic acid target. This method involves the use of RNA polymerase to synthesize RNA from a DNA template, leading to a significant increase in the amount of target RNA. This amplification can then be further analyzed or detected using various techniques.

Target amplification methods are crucial because they enable researchers and clinicians to detect low-abundance nucleic acids, making them essential in applications like viral load testing or the detection of pathogens in clinical samples. By amplifying the target, transcription-based methods ensure that there is enough material to analyze, allowing for increased sensitivity and specificity in molecular diagnostics.

In contrast, gel electrophoresis serves as a separation technique, isolation techniques focus on obtaining specific molecules from a mixture, and sequencing is used to determine the exact sequence of nucleotides in a DNA or RNA molecule. While all these methods are important in molecular biology, they do not fall under the category of target amplification methods like Transcription-Based Amplification does.