Which of the following is not a use for spectrophotometry?

Spectrophotometry is a widely utilized technique in molecular biology for measuring the absorbance of light by a sample at specific wavelengths. It is particularly useful in various applications where absorption characteristics of biomolecules are important.

Determining the optimal pH for absorbance of samples is a valid use of spectrophotometry. The absorbance of a solution can change with pH, impacting the ionization of certain functional groups within a molecule, ultimately affecting how the sample interacts with light.

Similarly, determining concentrations of unknown samples is a core application of spectrophotometry. By measuring the absorbance and applying Beer's Law, which relates absorbance to concentration, one can ascertain the concentration of an unknown substance based on a standard curve.

Identifying the optimal wavelength for absorbance is also relevant because different compounds absorb light at specific wavelengths. Spectrophotometry assists researchers in determining these optimal wavelengths to maximize sensitivity and specificity for their analyses.

In contrast, sequencing nucleic acids primarily involves techniques such as Sanger sequencing or next-generation sequencing methods, which do not rely on spectrophotometry for the sequencing process itself. While spectrophotometry can be used to quantify nucleic acids or assess their quality prior to sequencing, it is not directly employed in the