Which process is used in conjunction with isothermal amplification in hybrid capture?

Hybrid capture is a technique that involves the use of immobilized DNA probes to bind specifically to RNA targets. This method is typically combined with isothermal amplification to increase the sensitivity and specificity of the detection of specific nucleic acid sequences. By using immobilized probes, the targets can be captured from a sample, allowing for a more effective amplification process.

The process of hybrid capture ensures that only the intended RNA sequences are present for amplification, which enhances the overall performance of assays such as those used in diagnostics or gene analysis.

In contrast, options like capillary electrophoresis, fluorescent detection, and Western blotting are not directly related to the hybrid capture mechanism. While they may be useful in other biosensing techniques or analysis methods, they do not serve as the complementary process specifically paired with isothermal amplification in hybrid capture strategies. Thus, the use of immobilized DNA probes is central to the principle of hybrid capture in this context.