Which two viruses can be detected using hybrid capture alongside HPV?

The selection of Hepatitis B virus (HBV) and Cytomegalovirus (CMV) as viruses detectable alongside HPV using hybrid capture technology is based on the capabilities of hybrid capture assays. This method is particularly useful for identifying specific nucleic acid sequences within a mixture, which is essential for the diagnosis of various viral infections.

Hybrid capture assays utilize the principle of hybridization, where DNA or RNA from the target virus is captured with a labeled probe and then detected. Both HBV and CMV have well-characterized nucleic acids that can be efficiently targeted through this technique. HBV, being a major cause of liver disease, and CMV, which can lead to significant morbidity, especially in immunocompromised individuals, are important pathogens that benefit from this detection method.

In contrast, the other options include viruses that are generally not detected together with HPV using hybrid capture. HIV and HCV, while also significant viruses, are typically tested through different molecular methods, mainly due to their different clinical contexts and infection pathways. Influenza and RSV are respiratory viruses that are not usually associated with HPV infections, and detection methods used for them focus on respiratory samples and different viral particle characteristics. Norovirus and Adenovirus also employ different detection techniques and would not